SubtiBank SubtiBank
lytC [2017-05-24 13:47:35]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

lytC [2017-05-24 13:47:35]

N-acetylmuramoyl-L-alanine amidase, required for flagellar function
locus
BSU35620
pI
10.11
mw
52.46 kDa
protein length
496 aa Sequence Blast
gene length
1488 bp Sequence Blast
function
major autolysin, cell separation, wall turnover
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
cwlB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.4|Motility and chemotaxis/ other]

    • Gene

    Coordinates
    3,659,119 → 3,660,609

    Phenotypes of a mutant

  • impaired in motility, the phenotype is suppressed by mutations in ''[gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]'' or ''[gene|A6E7212D159F076F5D26F9C02F340B40C3667623|smiA]'' [Pubmed|19542270]

    • The protein

    Catalyzed reaction/ biological activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

    • Protein family

  • N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

    • [SW|Domains]

  • contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|CwlC], [protein|B09FB626274B81F00A4FB42D188E089095343182|CwlD], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|YqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|YrvJ])

    • Structure

  • [PDB|5J72] (from Clostridium difficile, 32% identity)

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|497C9654DC7514FF738D18E1F083299C43003A4C|lytA]-[gene|E1F9922DF7273040F84E5AC207A9F4CAFB85DC75|lytB]-[gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]
    description
    [Pubmed|1357079]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1357079], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|1357079], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: repression, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]
  • [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR]: repression, (in complex with [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]) [Pubmed|20351052], in [regulon|920F91E748EE079FF864011D9052B073567C41E4|SlrR regulon]
    • view in new tab

    Biological materials

    Mutant

  • 1A789 ( ''lytC''::''kan''), [Pubmed|1588906], available the [http://bgsc.org/getdetail.php?bgscid=1A789 Bacillus Genetic Stock Center]
  • BKE35620 (''lytC''::''erm trpC2'') is available at the [http://bgsc.org/ Bacillus Genetic Stock Center] [pubmed|28189581]

    • References

  • 19542270,10945275,14594841,1357079,16306698,20351052,9158733,27118079